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Methylene Blue Manufacturers USP BP Ph Eur Grade

Anmol Chemicals is a manufacturer supplier exporter of Methylene Blue and it offers materials as per IP BP EP Ph Eur USP NF JP FCC Food Grade as per the the latest monograph at best prices. Anmol Chemicals Group, established in 1976, is the pioneer manufacturer of Specialty Chemicals, Pharmaceutical Excipients, Some API, Food Chemicals in India. Anmol Chemicals Group has manufacturing facilities spread across Western India, representatives in Houston, Chicago USA and Dubai, UAE. We also have toll manufacturing units for processing chemicals in a few countries around the world. We make IP, BP, EP, USP, NF, Ph. Eur., JP, FCC or Food Grade, Analytical Reagent Grade, LR or Laboratory Reagent Grades and Pure Grades of various chemicals. All our items are analyzed to meet the required standards. We can supply the product in grams for your laboratory trial and in tons for your plant scale jobs.

We manufacture Bulk Drugs / API, Excipients, Pharmaceuticals (IP BP USP NF Ph Eur EP JP), Specialty Chemicals (Pure/Reagent), Mineral Fortifiers (FCC). Our manufacturing facility is FDA approved and GLP, cGMP, ISO9001, ISO14001, ISO/IEC 17025, ISO22000, FSSC 22000, ISO45001, FSSAI, Kosher, HALAL, COPP, WHO-GMP certified and Written Confirmation (WC) is available. Solid materials can be customized for particle size, shape, and bulk density. We observe WHO Good Manufacturing Practices and Good Laboratory Practices. We are a government-recognized STAR Export House and "Authorised Economic Operator (AEO)" per Indian Customs.

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Methylene Blue SDS of Manufacturers

Methylene Blue USP BP Manufacturers

Methylene Blue USP Grade Specifications

Methylene Blue
C16H18ClN3S-3H2O -- 373.90
Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride, trihydrate.
C.I. Basic Blue 9 trihydrate [CAS 7220-79-3]
Anhydrous 319.86 [CAS 61-73-4]

Methylene Blue contains not less than 98.0 percent and not more than 103.0 percent of C16H18ClN3S, calculated on the dried basis.
Identification: Infrared Absorption 197K .

Loss on drying— Dry it at 75 and at a pressure not exceeding 5 mm of mercury for 4 hours: it loses between 8.0% and 18.0% of its weight.
Residue on ignition: not more than 1.2%.
Arsenic— Prepare the Test Preparation by mixing 0.375 g with 10 mL of water in the arsine generator flask. Add 15 mL of nitric acid and 5 mL of perchloric acid, mix, and heat cautiously to the production of strong fumes of perchloric acid. Cool, wash down the sides of the flask with water, and again heat to strong fumes. Again cool, wash down the sides of the flask, and heat to fumes. Cool, dilute with water to 52 mL, and add 3 mL of hydrochloric acid: the resulting solution meets the requirements of the test, the addition of 20 mL of 7 N sulfuric acid specified for Procedure being omitted. The limit is 8 ppm.
Copper or zinc— Ignite 1.0 g in a porcelain crucible, using as low a temperature as practicable, until all of the carbon is oxidized. Cool the residue, add 15 mL of 2 N nitric acid, and boil for 5 minutes. Filter the cooled solution, and wash any residue with 10 mL of water. To the combined filtrate and washing add an excess of 6 N ammonium hydroxide, and filter the solution into a 50 mL volumetric flask. Wash the precipitate with small portions of water, adding the washings to the filtrate, dilute the solution with water to volume, and mix. To 25 mL of the solution add 10 mL of hydrogen sulfide TS: no turbidity is produced within 5 minutes (absence of zinc). Any dark color produced does not exceed that of a control prepared by boiling a quantity of cupric sulfate, equivalent to 200 @g of copper, with 15 mL of 2 N nitric acid for 5 minutes and by treating this solution as directed above, beginning with “Filter the cooled solution” (0.02% of copper).
Chromatographic purity— Quantitatively dissolve an accurately weighed quantity of Methylene Blue in methanol to obtain a Test solution containing 1.0 mg per mL Dissolve a suitable quantity of USP Methylene Blue RS in methanol to obtain a Standard solution having a concentration of 100 @g per mL. Quantitatively dilute a portion of this solution with methanol to obtain a Diluted standard solution having a concentration of 10 @g per mL. Apply 5 @L each of the Test solution, the Standard solution, and the Diluted standard solution to a suitable thin-layer chromatographic plate coated with a 0.25-mm layer of octadecylsilanized chromatographic silica gel. Allow the spots to dry, and develop the chromatogram in a chromatographic chamber with a solvent system consisting of a mixture of the upper layer separated from a well-shaken mixture of water, n-butanol, and glacial acetic acid (100:80:20), until the solvent front has moved about three-fourths of the length of the plate. Remove the plate from the chamber, allow the solvent to evaporate, and visually locate the spots on the plate: the RF value of the principal spot in the chromatogram from the Test solution corresponds to that from the Standard solution, and other spots, if present in the chromatogram from the Test solution, consist of a secondary spot that does not exceed in size or intensity, the principal spot obtained from the Standard solution (10%), and not more than two additional spots, neither of which exceeds in size or intensity the principal spot from the Diluted standard solution (1%)Assay— Transfer about 100 mg of Methylene Blue, accurately weighed, to a 250-mL volumetric flask, dissolve in and dilute with diluted alcohol to volume, and mix. Transfer 5.0 mL of this solution to a 100-mL volumetric flask, dilute with diluted alcohol to volume, and mix. Transfer 5.0 mL of this solution to a 50-mL volumetric flask, dilute with diluted alcohol to volume, and mix. This solution contains about 2 @g per mL. Dissolve an accurately weighed quantity of USP Methylene Blue RS in diluted alcohol, and dilute quantitatively and stepwise with diluted alcohol to obtain a Standard solution having a known concentration of about 2 @g per mL. Concomitantly determine the absorbance of both solutions in 1-cm cells at the wavelength of maximum absorbance at about 663 nm, with a suitable spectrophotometer, using diluted alcohol as the blank. Calculate the quantity, in mg, of C16H18ClN3S in the Methylene Blue taken by the formula:

50C(AU / AS)

in which C is the concentration, in @g per mL, of anhydrous methylene blue in the Standard solution; and AU and AS are the absorbance of the solution of Methylene Blue and the Standard solution, respectively.

Keywords: Methylene Blue IP BP Ph Eur EP USP Analytical Reagent FCC Food Grade trihydrate CAS 7220-79-3 anhydrous CAS 61-73-4 Manufacturer Supplier Exporter Monograph Uses Chemical Molecular Formula Weight.

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Manufacturers

ANMOL CHEMICALS
Now maturing as Anmol Chemicals Private Limited
Representatives in Houston, Chicago and New York, USA
TEL: (OFF) +91 22 23726950, +91 22 23774610, +91 22 23723564
Taloja Navi Mumbai, INDIA

e-mail: info@anmol.org

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Last updated 11-mar-24

Calcium Chloride in Jumbo Bags Drum Packing Paletized and Shrink Wrapped Packing for Exports to USA UAE Africa Tanzania Turkey Kenya Dubai

Exporters to USA, Canada, UAE, Kuwait, Qatar, Gautemala, Tunisia, Europe, South Africa, Bangladesh, Sri Lanka, Tanzania, Kenya, Egypt, Iraq, Turkey, Israel, Vietnam, Nigeria, Uganda, Brazil, Chile, Argentina, Dubai, UAE, Saudi Arabia, Cameroon, Mexico, Brazil, Chile, Argentina, Korea, Thailand, Malaysia, Indonesia, Jakarta, Australia, China, Germany, France, etc.

Representatives in New York, Houston - Texas, Chicago - Illinois, Los Angeles.

All products are Sold for Industrial and Laboratory Use only. No other use is permitted.

Fast Selling IP BP Ph Eur EP USP NF JP Analytical Reagent FCC Food Grades of Chemicals by Anmol Chemicals
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Methylene Blue
IP BP USP Grade Manufacturers
Methylene Blue

Methylene Blue is also called ( CAS No. 548-62-9; 603-47-4 )
Basic blue 9 (C.I. 52015)
3,7-Bis-(dimethylamino)-phenazathonium chloride
3,7-Bis-(dimethylamino)-5-phenothiazinium chloride
C.I. 52015
Methylene blue (C.I. 52015)
Solvent blue 8 (C.I. 52015)
Swiss blue
Tetramethylthionine chloride
Urolene blue